WebDTT buffer: 0.1 M Tris/H 2 SO 4 pH 9.4, 10 mM DTT freshly added prior to use from a 1 M stock in H 2 O; stored in aliquots at − 20°C. Zymolyase buffer: 20 mM KPi pH 7.4, 1.2 M sorbitol. Homogenization buffer (pre-cooled to 4°C): 0.6 M sorbitol, 10 mM Tris/HCl pH 7.4, 1 mM ethylenediaminetetraacetic acid (EDTA), 1 mM phenylmethylsulfonyl fluoride … WebBioBasic SEC 60 60Å 0.7ccg-1 5µm BioBasic SEC 120 120Å 1.0ccg-1 5µm BioBasic SEC 300 300Å 0.9ccg-1 5µm BioBasic SEC 1000 1000Å 0.9ccg-1 5µm How Retention is Achieved In size exclusion, retention is deter-mined by the accessibility of the sample molecule to the pores. Maxi-mum retention occurs if the sample can fully access the …
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WebBio Basic challenges the preconceptions that good science is dependent upon expensive research products. We hope that the quality of our products and services is able to carve … WebMar 9, 2024 · All protein purification steps were performed on ice or at 4 °C. Thawed cell pellets were resuspended with a handheld homogenizer (Polytron PT1200E) in a solution of 300 mM NaCl, 50 mM HEPES pH 8.0, 2 mM MgCl 2 2, 2 mM DTT, and 5% glycerol. PreScission protease was added to the column at a concentration of 0.35 mg/mL for 3 h … how many horsepower does a smart car have
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