Diacetylchitobiose deacetylase
WebAug 14, 2024 · Diacetylchitobiose deacetylase has great application potential in the production of chitosan oligosaccharides and monosaccharides. This work aimed to achieve high-level secretory production of diacetylchitobiose deacetylase by Bacillus subtilis and perform molecular engineering to improve catalytic performance. First, we screened 12 … WebAug 14, 2024 · Diacetylchitobiose deacetylase has great application potential in the production of chitosan oligosaccharides and monosaccharides. This work aimed to …
Diacetylchitobiose deacetylase
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WebDec 1, 2024 · Diacetylchitobiose deacetylase Expression element optimization Cell factory Bacillus subtilis Protein secretion 1. Introduction Glucosamine (GlcN) plays an … WebConcerted Action of Diacetylchitobiose Deacetylase and Exo- -D-glucosaminidase in a Novel Chitinolytic Pathway in the Hyperthermophilic Archaeon Thermococcus kodakaraensis KOD1* Received for publication, December 26, 2003, and in revised form, May 3, 2004 Published, JBC Papers in Press, May 10, 2004, DOI 10.1074/jbc.M314187200
WebJul 16, 2004 · The hyperthermophilic archaeon Thermococcus kodakaraensis KOD1 possesses chitinase (Tk-ChiA) and exo-β-d-glucosaminidase (Tk-GlmA) for chitin degradation; the former produces diacetylchitobiose (GlcNAc 2) from chitin, and the latter hydrolyzes chitobiose (GlcN 2) to glucosamine (GlcN).To identify the enzyme that … WebNov 7, 2013 · In order to develop a structure-based understanding of the chitinolytic pathway in hyperthermophilic Pyrococcus species, we performed crystallographic studies on N,N'-diacetylchitobiose deacetylases (Dacs) from Pyrococcus horikoshii (Ph-Dac) and Pyrococcus furiosus (Pf-Dac). Neither Ph-Dac nor Pf-Dac was expressed in the soluble …
WebJun 16, 2012 · N,N'-diacetylchitobiose deacetylase (Dac) belongs to the CE-14 family and plays a role in the chitinolytic pathway in archaea by deacetylating N,N'-diacetylchitobiose (GlcNAc2), which is the end ... WebJan 1, 2024 · Diacetylchitobiose deacetylase 1. Introduction Sorting cells of interest are fundamental to synthetic biology, clinical research, drug discovery, and other fields ( Gach et al., 2024; Heath et al., 2016; Liu et al., 2024; Sivaramakrishnan et al., 2024; Sun et al., …
WebDiacetylchitobiose deacetylase2-acetamido-2-deoxy-beta-D-glucopyranoseZINC ION. NCBI. National Center for Biotechnology Information. Search. 8BGO: N,N-diacetylchitobiose deacetylase from Pyrococcus chitonophagus with substrate N,N-diacetylchitobiose. PDB ID: 8BGO Download: MMDB ID: 225224: PDB Deposition …
WebAug 24, 2024 · This deacetylase can also use diacetylchitobiose-6-phosphate as a substrate to produce monoacetylchitobiose-6-phosphate, which is the inducer and substrate of ChbR and ChbF, respectively 14. greening of corporate americaWebWe constructed a recombinant Escherichia coli and Bacillus subtilis strains as efficient whole-cell biocatalysts via expression of diacetylchitobiose deacetylase (Dacph) from Pyrococcus horikoshii. Although both strains were biocatalytically active, the performance of B. subtilis was better. flyer invitation foireWebJul 1, 2013 · The purified Pyrococcus Dacs were active and thermostable up to 85 °C. The crystal structures of Ph-Dac and Pf-Dac were determined at resolutions of 2.0 Å and 1.54 Å, respectively. The Pyrococcus Dac forms a hexamer composed of two trimers. These Dacs are characterized by an intermolecular cleft, which is formed by two polypeptides in the ... flyer introducing new employeeWebAug 4, 2024 · Gene ID: 13302188, updated on 4-Aug-2024. Summary Other designations. PIG-L family deacetylase. GeneRIFs: Gene References Into Functions. Crystal structure of N,N'-diacetylchitobiose deacetylase from Pyrococcus furiosus has been reported. greening of arabia prophecygreening of forsyth fountainWebThe hyperthermophilic archaeon Thermococcus kodakaraensis KOD1 possesses chitinase (Tk-ChiA) and exo-β-d-glucosaminidase (Tk-GlmA) for chitin degradation; the former produces diacetylchitobiose (GlcNAc2) from chitin, and the latter hydrolyzes chitobiose (GlcN2) to glucosamine (GlcN). To identify the enzyme that physiologically … greening of america bookWebSelect search scope, currently: articles+ all catalog, articles, website, & more in one search; catalog books, media & more in the Stanford Libraries' collections; articles+ journal articles & other e-resources greening of ghent 2023